| 质粒名称: | pGL3-(2 BsaI)-PGK-puroR-mRFP1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002187 |
| 产品用途: | The expression vector containing RFP was used for co-expression of hCtRNA-driven pegRNA and nicking RNA. |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PGK |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PGK-puroR-mRFP1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pGL3-(2 BsaI)-PGK-puroR-mRFP1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pGL3-(2 BsaI)-PGK-puroR-mRFP1载体质粒基本信息
pGL3-(2 BsaI)-PGK-puroR-mRFP1质粒图谱载体图谱和pGL3-(2 BsaI)-PGK-puroR-mRFP1载体序列质粒序列多克隆位点信息
-PGK-puroR-mRFP1载体图谱.png)
pGL3-(2 BsaI)-PGK-puroR-mRFP1质粒载体简介
pGL3-(2 BsaI)-PGK-puroR-mRFP1质粒序列载体序列
LOCUS pGL3-(2 BsaI)-PGK-puroR-mRFP1 5323 bp DNA circular SYN 06-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5323)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5323
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(21..48)
/label=OL459
primer_bind 37..61
/label=OL441
primer_bind complement(41..65)
/label=OL442
primer_bind 55..81
/label=OL458
misc_feature 100..217
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 266..776
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind complement(360..379)
/label=OL1068
primer_bind 550..566
/label=OL446
primer_bind 695..713
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 798..1394
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(798..817)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 1294..1314
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 1365..1419
/label=OL901
primer_bind complement(1371..1394)
/label=OL306
primer_bind complement(1380..1402)
/label=OL384
primer_bind 1387..1418
/label=OL379
CDS 1395..1451
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
primer_bind 1395..1451
/label=OL866
primer_bind complement(1395..1451)
/label=OL867
primer_bind 1432..1475
/label=OL902
primer_bind complement(1432..1459)
/label=OL966
RBS 1434..1441
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 1452..2129
/codon_start=1
/product="monomeric derivative of DsRed (Campbell et al.,
2002)"
/label=mRFP1
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
TGA"
primer_bind complement(2107..2154)
/label=OL903
primer_bind 2128..2146
/label=OL965
primer_bind 2130..2152
/label=OL305
polyA_signal 2276..2397
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2287..2306)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2341..2360
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2586..2609)
/label=OL1043
primer_bind 2587..2609
/label=OL1044
primer_bind complement(2645..2662)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2816..3404)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2896..2915)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(2998..3017)
/label=OL1041
primer_bind complement(3131..3150)
/label=OL631
primer_bind 3187..3206
/label=OL798
primer_bind complement(3428..3467)
/label=OL660
primer_bind 3448..3467
/label=OL659
CDS complement(3575..4435)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4198..4217
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
primer_bind 4351..4370
/label=OL1033
primer_bind complement(4351..4370)
/label=OL1034
promoter complement(4436..4540)
/gene="bla"
/label=AmpR promoter
rep_origin 4567..5022
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4654..4673)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4864..4885
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 5153..5201
/note="synthetic polyadenylation signal"
misc_feature 5215..5306
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 5255..5274
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 agtgaacgga tctcgacggt atcgatcacg agactagcct tgagaccgag agagggtctc
61 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
121 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
181 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
241 agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg
301 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
361 ctgggactcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
421 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
481 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
541 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
601 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
661 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
721 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg
781 atccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
841 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
901 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
961 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
1021 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
1081 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
1141 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
1201 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
1261 gggtgcccgc cttcctggaa acctccgcgc cccgcaacct ccccttctac gagcggctcg
1321 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
1381 gcaagcccgg tgccgctact aacttcagcc tgctgaagca ggctggagac gtggaggaga
1441 accctggacc tatggcgagt agcgaagacg ttatcaaaga gttcatgcgt ttcaaagttc
1501 gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc
1561 cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg
1621 cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg
1681 ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta
1741 tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg
1801 agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc
1861 agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc
1921 tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag
1981 ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca
2041 tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg
2101 ctgaaggtcg tcactccacc ggtgcttaac gcccgcccca cgacccgcag cgcccgaccg
2161 aaaggagcgc acgaccccat gcatcggtac ctttaagacc aatgacttac aaggcagctg
2221 tagatcttag ccactttcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
2281 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
2341 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
2401 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
2461 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt
2521 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
2581 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
2641 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
2701 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
2761 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
2821 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
2881 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
2941 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
3001 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
3061 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
3121 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
3181 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
3241 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
3301 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
3361 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
3421 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
3481 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
3541 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
3601 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
3661 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgggacc
3721 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
3781 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
3841 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
3901 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
3961 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
4021 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
4081 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
4141 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
4201 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
4261 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
4321 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
4381 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
4441 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
4501 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
4561 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
4621 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
4681 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
4741 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
4801 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
4861 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
4921 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
4981 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttgccattcg ccattcaggc
5041 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcccaagc
5101 taccatgata agtaagtaat attaaggtac gggaggtact tggagcggcc gcaataaaat
5161 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata
5221 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc
5281 aagtgcaggt gccagaacat ttctctatcg ataggtaccg att
//
